Name
of the technique
Fluid
used
Parts
of the pipette
Gradation
in the pipette
Amount
of fluid in pipette
Bead
color
Function
of bead
Anticoagulant
used and Percentage
Which
Vein used to collect blood – other areas
Name
of the chamber used
Name
of diluting fluid
Composition
of fluid and function of each component
ml
of blood used
Normal
range in
Adult
Male
Adult
Female
Lactating
females
Pregnant
females
New
born
Infant
Child
below 6years,
Children
6 -12 yrs of age.
Causes
for increase in total count
Physiological
Pathological
Causes
for decrease in total count
Physiological
Pathological
Radiological,
clinical manifestation of deficiency of each type of cells
Oral
manifestation of deficiency with pathophysiology of each cells
Distribution
of each type of cell
Structure,
dimensions & function of each type of cell
Life
period of each type of cell
Synthesis
and differentiation of each cell
Enzymes
& Hormone involved in production of cells
Staining
characteristics
Other
methods of counting DC
Manual
Automated
Principles
of other methods
What
errors could lead to false count during & how to rectify
Blood
collection
Storage
Loading
in pipette
Loading
the chamber
Coverslip
placement
Count
Function
of each type of cell
Site
of production
Absolute
counts
What
is
Leukocytosis
Leukopenia
Neutropenia
Agranulocytosis
Neutrophilia
Eosinophilia
Granules
characteristics & composition of
Eosinophils
Neutrophils
Monocytes
Platelet
count
INR
PTT
PT
BT
CT
Relevance
of following to blood disorders
SGOT
SGPT
Acute
inflammation changes in DC
Chronic
inflammation changes in DC
Cell
count changes in HIV
CD4:CD8
Ratio
Stabs
Shift
to right
Shift
to left
Basopenia
Eosinopenia
Drugs
which increase the number of basophils:
Drugs
which decrease the number of basophils:
Drugs
which increase the number of eosinophils:
Drugs
which decrease the number of eosinophils:
Drugs
which decrease the number of lymphocytes:
Drugs
which decrease the number of monocytes:
Drugs
which increase the number of neutrophils:
Drugs
which decrease the number of neutrophils:
Thromobocytosis
Thrombocytopenia
Platelet
normal values
Megakaryocytes
Conditions
in which platelet count is increased:
Condition
in which platelet count is decreased:
Drugs
which may increase the platelet count:
Drugs
which may decrease the platelet count:
What
is the rationale, principle and normal range for the following tests:
ANEMIA
DIAGNOSIS
Blood
smear
Complete
blood cell count
Ferritin
Folic
acid
Free
erythrocyte protoporphyrin
Glucose-6-phosphate
dehydrogenase
Iron
RBC
indices
RBC
distribution width
Reticulocyte
count
Schilling
test
Total
iron-binding capacity
Transferrin
Transferrin
saturation
Vitamin
B12
COAGULATION
STUDIES
Anti-thrombin
III
Bleeding
time
Coagulation
factor assay
D-dimer
test
Euglobulin
lysis time
Fibrin
degradation products
Fibrinogen
Partial
thromboplastin time
Plasminogen
Protein
C
Protein
S
Prothrombin
time
Thrombin
clotting time
PLATELET
ACTIVITY TESTS
Bleeding
time
Platelet
aggregation test
Platelet
count
Platelet,
mean volume