Wave length of light
Spectrum of
electromagnetic energy, light
electromagnetic energy, light
Wavelength of
Infrared
Ultraviolet
Electron
X rays
Gamma rays
Laser – commonly used
ones
ones
Define:
Monochromatic
Polychromatic
Color temperature
Emission spectrum
Amplitude
Intensity
Cadescence
Reflection
Refraction
Retardation
Angle of incidence
Angle of refraction
Refractive index
Curvature of lens
Concave lens
Convex lens
Real image
Virtual image
Total internal reflection
Principal focus
Focal length of a lens
Conjugative foci
Chromatic aberration
Achromat
Apochromat
Plan apochromats
Semi-apochromatic
CF optics
Spherical aberrations
Euchromatic
Filters
Neutral density filters
Numerical aperture
Condensers
Iris diaphragm
Glare
Resolution
Centration of condenser
“Swing out top lens” of
the condenser
the condenser
Infinity corrected
objectives
objectives
Magnifying power
Resolving power
Nose piece
Par-focal
Par-central
Beam splitting prism
Tube lens
Infinity corrected lens
Periplanatic lens
Wide field lens
High focal point lens
& their use
& their use
Magnification values
& formula for it
& formula for it
Empty magnification
Critical illumination
Kohler illumination
Rheinberg illumination
Back focal plane
Constructive interference
Destructive interference
Phase halo
Optical path difference
Optical weighing
Measuring and reference
beam in interference microscopy
beam in interference microscopy
Birefringence
Differential interference
contrast
contrast
Plane of vibration
Dichorism
Pleochoric
Primary fluorescence or
auto fluorescence
auto fluorescence
Flurochromes
Induced flurosomes
Suppression filters
Reflected light
Transmitted light
Visibility
Contrast
Depth of field
Working distance
What is Refractive
index of
index of
Glass
Water
Air
Universal Color
code of
code of
Objective
2 X
4X
10X
20X
40X
100X
4.
Diameter
of Condenser – fully opened & closed
Diameter
of Condenser – fully opened & closed
5.
Function
of Vernier markings on the stage
Function
of Vernier markings on the stage
6.
Maximum
Numerical Aperture attainable
Maximum
Numerical Aperture attainable
7.
Number
of nose pieces
Number
of nose pieces
8.
Huyghens
eye piece design
Huyghens
eye piece design
Bertrands lens
Ramsden & Kellner eye
piece
piece
Compensating eyepieces
9.
Principle
behind
Principle
behind
Dark field microscopy
Phase contrast microscopy
Interference microscopy
Fluorescence microscopy
Confocal Microscopy
FRET
Multispectral Imaging
Scanning Electron
microscopy
microscopy
Transmission Electron
microscopy
microscopy
10.
Nicol
Prism(Calcite cemented with Canada Balsm after Nicol)
Nicol
Prism(Calcite cemented with Canada Balsm after Nicol)
Wollaston prism
11.
Length
of the tube
Length
of the tube
12.
Dimension
of (LX B x H)
Dimension
of (LX B x H)
Slides
Coverslip
13.
Describe
Describe
Lint free slides
Coated slides
Frosted slides
Substage condenser lens
Substage iris diaphragm
Daylight glass
Field of view
Field
lens
lens
14.
Microscope
oil – Why, what, when, how, precaution
& trouble shooting
Microscope
oil – Why, what, when, how, precaution
& trouble shooting
Maintenance
15.
Lens
paper, Cleaning agents for microscope,
Lens
paper, Cleaning agents for microscope,
Fungal growth
16.
Resolution
of
Resolution
of
Naked eye
Light microscopy
Electron microscopy
Confocal microscopy