List

Stage Viva in Staining for MDS – Oral Pathology students

 December 5, 2013 |  admin |  Lecture series, Faculty Development Program Series

1.     
Thickness
of section
2.     
Serial
Section
3.     
Artifacts
in sections
4.     
Types
of Hematoxylin and eosin
5.     
Principle
of Hematoxylin
6.     
Oxidation
(Natural, Chemical) Ripening of Hematoxylin
7.     
Fixative
– Types, principles, components
8.     
Bluing
, Reverse bluing
9.     
Mordants
, common mordants
10. 
Charge
of Hematoxylin
11. 
Classification
of Hematoxylin
12. 
Advantage
and disadvantage of various types of Hematoxylin
13. 
Esoin
belongs to _________ Class of dyes
14. 
End
point determination of decalcification
15. 
Classify
decalcification agents
16. 
Medavars
law
17. 
Properties
of ideal fixatives
18. 
Ideal
fixation timing
19. 
Common
agents for fixations
20. 
pH,
Temperature of fixation
21. 
penetration
of fixants, methelyne glycol and carbonyl form
22. 
Why
alcohol and xylene are used before paraffin embedding
23. 
Melting
point of paraffin wax
24. 
Miscibility
of a solution 
25. 
Idea
behind fixation
26. 
Cross
linking of proteins, denaturation of proteins
27. 
Antigen
retrival – methods and principle
28. 
Types
of microtomes
29. 
Principle
behind each type of microtomes
30. 
Blades
– types, mechanical
31. 
Horning
and strapping
32. 
Formalin
pigments – identification & removal
33. 
Post
fixation treatments
34. 
Tissue
cassette dimensions, material,  
35. 
What
is
Dehydration
Clearing
Embedding media
Section adhesive – common
ones, composition
Antiroll plate
Quenching
Thawing
Special stains
Fluorescent staining
IHC
36. 
Conditions
for a water bath, hot plate for section cutting – temperature
37. 
Frozen
sections, cryostats, frozen section staining
38. 
Enzyme
histochemistry
39. 
Mountants
– curing time, type and specific advantage of each
40. 
Alternatives
for paraffin wax

41. 
Fixatives
for EM studies