|
The technique that provides
more exact in situ information about chemical composition of cells |
Histochemical techniques
|
|
The biochemical probe that best
characterizes sugar moieties with glycoproteins |
Lectins
|
|
The examples of histochemistry
quantitative analysis |
Microphotocell counter; Double
beam recording microdensiometry; Integrating microdensiometry |
|
The techniques that use both
quantitative and qualitative analysis of tissue substances |
Laser spectroscopy; X-ray
spectrophotometry; Electron probe microanalysis |
|
Radioautographic techniques
|
A technique that elucidate the
uptake of chemical substance by different metabolic pathways of different tissues and regions of cytoplasm |
|
Proteoglycans are characterized
by |
Large molecule; Has a protein
core; Large number of glycosaminoglycans attached and composed of non-branching disaccharide units |
|
Glycoproteins are characterized
|
Has a protein macromolecule
core; Fewer carbohydrate moieties;Composed of branching disaccharide units |
|
Laminin is secreted
|
Epithelial cells
|
|
During inflammation / early
stages of wound healing there is a histochemically demonstrable |
Increase in glycoproteins and
proteoglycans |
|
Deficiency of ascorbic acid
causes |
Loss of collagen molecular
stability; Loss of stability of triple of helix; Abnormal immature collagen |
|
Histochemical identification of
mucins in salivary glands are based on |
Glycosaminoglycan content
|
|
Formaldehyde when used as a
fixative, it’s ideal concentration is |
10%
|
|
The commonly used fixative for
electron microscopy is |
Glutaldehyde
|
|
The premise of protein based
antigens bind to specific antibodies in employed in |
Immunohistochemistry
|
|
The presence of a bluish blue
opalescence in adult teeth of dentinogenesis imperfecta is associated with localization of |
Type III collagen
|
|
Developing molars exhibited
alkaline phosphatase activity in |
Stratum intermedium
|
|
In electron microscopy the
lower penetrating power of electron beam necessitates |
Difference in tissue
preparation |
|
The most commonly used method
of tissue preparation is |
Embedding in paraffin
|
|
The thickness of sections cut
using paraffin embedded tissue is in the range of |
4 to 10 micrometers
|
|
The thickness of ground
sections is in the range of |
About 50 micrometers
|
|
The most commonly used dental
fixative is |
10% neutral formalin
|
|
Fixation of tissues helps to
|
Coagulate the protein; Prevents
decomposition; Increases permeability |
|
The time required for fixation
of a human tissue specimen is a factor(s) of |
Size of tissue; Density of
specimen; Type of fixative solutions |
|
The aim of dehydration is to
|
Substitute water with alcohol
so paraffin to diffuse into the tissue |
|
While dehydration of tissue
during processing, initially the tissue is subjected to |
Increasing percentage of
alcohol |
|
The processing solution that is
miscible with paraffin is |
Xylene
|
|
The Meyers adhesive is made of
|
Egg albumin and glycerin
|
|
To study pulp it should be
ideally fixed with the fixative |
Apex opened and immersed in
fixative |
|
The ways to determine end point
of decalcification are |
Piercing with needle;
Precipitation test; Radiographic method |
|
The best way to determine end
point of decalcification is |
Precipitation test
|
|
Blocks of parlodion should
always be stored in |
Alcohol
|
|
Parlodion is purified
|
Nitrocellulose in ether-
alcohol |
|
Parlodion embedded sections are
cut in |
Sliding microtome
|
Prof. Rooban Thavarajah
Oral and Maxillofacial Pathologist
List