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Histochem-1

 July 11, 2013 |  admin |  Lecture series, Faculty Development Program Series
The technique that provides
more exact in situ information about chemical composition of cells
Histochemical techniques
The biochemical probe that best
characterizes sugar moieties with glycoproteins
Lectins
The examples of histochemistry
quantitative analysis
Microphotocell counter; Double
beam recording microdensiometry; Integrating microdensiometry
The techniques that use both
quantitative and qualitative analysis of tissue substances
Laser spectroscopy; X-ray
spectrophotometry; Electron probe microanalysis
Radioautographic techniques
A technique that elucidate the
uptake of chemical substance by different metabolic pathways of different
tissues and regions of cytoplasm
Proteoglycans are characterized
by
Large molecule; Has a protein
core; Large number of glycosaminoglycans attached and composed of
non-branching disaccharide units
Glycoproteins are characterized
Has a protein macromolecule
core; Fewer carbohydrate moieties;Composed of branching disaccharide units
Laminin is secreted
Epithelial cells
During inflammation / early
stages of wound healing there is a histochemically demonstrable
Increase in glycoproteins and
proteoglycans
Deficiency of ascorbic acid
causes
Loss of collagen molecular
stability; Loss of stability of triple of helix; Abnormal immature collagen
Histochemical identification of
mucins in salivary glands are based on
Glycosaminoglycan content
Formaldehyde when used as a
fixative, it’s ideal concentration is
10%
The commonly used fixative for
electron microscopy is
Glutaldehyde
The premise of protein based
antigens bind to specific antibodies in employed in
Immunohistochemistry
The presence of a bluish blue
opalescence in adult teeth of dentinogenesis imperfecta is associated with
localization of 
Type III collagen
Developing molars exhibited
alkaline phosphatase activity in
Stratum intermedium
In electron microscopy the
lower penetrating power of electron beam necessitates
Difference in tissue
preparation
The most commonly used method
of tissue preparation is
Embedding in paraffin
The thickness of sections cut
using paraffin embedded tissue is in the range of
4 to 10 micrometers
The thickness of ground
sections is in the range of
About 50  micrometers
The most commonly used dental
fixative is
10% neutral formalin
Fixation of tissues helps to
Coagulate the protein; Prevents
decomposition; Increases permeability
The time required for fixation
of a human tissue specimen is a factor(s) of
Size of tissue; Density of
specimen; Type of fixative solutions
The aim of dehydration is to
Substitute water with alcohol
so paraffin to diffuse into the tissue
While dehydration of tissue
during processing, initially the tissue is subjected to
Increasing percentage of
alcohol
The processing solution that is
miscible with paraffin is
Xylene
The Meyers adhesive is made of
Egg albumin and glycerin
To study pulp it should be
ideally fixed with the fixative
Apex opened and immersed in
fixative
The ways to determine end point
of decalcification are
Piercing with needle;
Precipitation test; Radiographic method
The best way to determine end
point of decalcification is
Precipitation test
Blocks of parlodion should
always be stored in
Alcohol
Parlodion is purified
Nitrocellulose in ether-
alcohol
Parlodion embedded sections are
cut in
Sliding microtome